Seeing is believing…
The Wong lab employs advanced fluorescence microscopy approaches—including high-resolution multiplexed imaging, cleared organ volumetric imaging, and dynamic two-photon intravital imaging—to study how immune responses are controlled across time and space in tissues.
Visualizing control of autoimmune T cell responses.
An entire murine gastric lymph node was optically cleared and imaged in 3D. Red = Endogenous regulatory T cells, Magenta = Self-reactive CD4+ T cells responding to an endogenous self-antigen, Green = Polyclonal control CD4+ T cells.
Homeostatic dynamics of regulatory T cells.
Two-photon intravital 4D imaging of regulatory T cells in the popliteal lymph node of a living mouse. Cyan = Endogenous regulatory T cells, Magenta = Polyclonal control T cells, Blue = Collagen fibres
Visualizing the lung-immune interface.
A thick murine lung section (200 um) was optically cleared and imaged in 3D. Purple = Type 2 alveolar cells, Green = Airway epithelium, Orange = T cells, Red = Alveolar macrophages, White = MHCII+ antigen-presenting cells